htsjdk.samtools.reference

Class FastaSequenceFile

java.lang.Object

htsjdk.samtools.reference.FastaSequenceFile

All Implemented Interfaces:

ReferenceSequenceFile, java.io.Closeable, java.lang.AutoCloseable

public class FastaSequenceFile
extends java.lang.Object

Implementation of ReferenceSequenceFile for reading from FASTA files.

Field Summary

Fields

Modifier and Type	Field and Description
protected java.io.File	file
protected SAMSequenceDictionary	sequenceDictionary

Constructor Summary

Constructors

Constructor and Description
FastaSequenceFile(java.io.File file, boolean truncateNamesAtWhitespace) Constructs a FastaSequenceFile that reads from the specified file.

Method Summary

Modifier and Type	Method and Description
void	close() It's good to call this to free up memory.
protected static java.io.File	findSequenceDictionary(java.io.File file)
ReferenceSequence	getSequence(java.lang.String contig) default implementation -- override if index is supported
SAMSequenceDictionary	getSequenceDictionary() Returns the list of sequence records associated with the reference sequence if found otherwise null.
ReferenceSequence	getSubsequenceAt(java.lang.String contig, long start, long stop) default implementation -- override if index is supported
boolean	isIndexed() default implementation -- override if index is supported
ReferenceSequence	nextSequence() Retrieves the next whole sequences from the file.
void	reset() Resets the ReferenceSequenceFile so that the next call to nextSequence() will return the first sequence in the file.
java.lang.String	toString() Returns the full path to the reference file.

Methods inherited from class java.lang.Object

clone, equals, finalize, getClass, hashCode, notify, notifyAll, wait, wait, wait

Field Detail

file

protected final java.io.File file

sequenceDictionary

protected SAMSequenceDictionary sequenceDictionary

Constructor Detail

FastaSequenceFile

public FastaSequenceFile(java.io.File file,
                         boolean truncateNamesAtWhitespace)

Constructs a FastaSequenceFile that reads from the specified file.

Method Detail

close

public void close()

It's good to call this to free up memory.

nextSequence

public ReferenceSequence nextSequence()

Description copied from interface: ReferenceSequenceFile

Retrieves the next whole sequences from the file.

Returns:

a ReferenceSequence or null if at the end of the file

reset

public void reset()

Description copied from interface: ReferenceSequenceFile

Resets the ReferenceSequenceFile so that the next call to nextSequence() will return the first sequence in the file.

findSequenceDictionary

protected static java.io.File findSequenceDictionary(java.io.File file)

getSequenceDictionary

public SAMSequenceDictionary getSequenceDictionary()

Returns the list of sequence records associated with the reference sequence if found otherwise null.

Specified by:

getSequenceDictionary in interface ReferenceSequenceFile

Returns:

a list of sequence records representing the sequences in this reference file

toString

public java.lang.String toString()

Returns the full path to the reference file.

Specified by:

toString in interface ReferenceSequenceFile

Overrides:

toString in class java.lang.Object

Returns:

Reference name, file name, or something other human-readable representation.

isIndexed

public boolean isIndexed()

default implementation -- override if index is supported

Specified by:

isIndexed in interface ReferenceSequenceFile

Returns:

true if getSequence and getSubsequenceAt methods are allowed.

getSequence

public ReferenceSequence getSequence(java.lang.String contig)

default implementation -- override if index is supported

Specified by:

getSequence in interface ReferenceSequenceFile

Parameters:

contig - contig whose data should be returned.

Returns:

The full sequence associated with this contig.

getSubsequenceAt

public ReferenceSequence getSubsequenceAt(java.lang.String contig,
                                          long start,
                                          long stop)

default implementation -- override if index is supported

Specified by:

getSubsequenceAt in interface ReferenceSequenceFile

Parameters:

contig - Contig whose subsequence to retrieve.

start - inclusive, 1-based start of region.

stop - inclusive, 1-based stop of region.

Returns:

The partial reference sequence associated with this range.